Inverted microscopes – extending the benefits of light microscope
Typically, a lab specimen is first made to undergo certain procedure to prepare it for observation. After going through the process, organism loses its life, which eventually produces inaccurate results. This is how it happens when using a conventional microscope. This disadvantage was overcome by light microscope.
What light microscope does is that it allows the user to analyze a microorganism while it is still alive, and observe its various living functions, which is not possible if the specimen is dead. The introduction of inverted microscopes extended the advantage of former one-step forward.
With traditional light microscopes, the specimen is first placed on the glass slide. Before the sample could be placed on the glass slide, a small part of it has to be first removed from the culture and placed in an unnatural environment of the glass slide and its slip.
Once the specimen is put there, its oxygen content begins to change quickly due to change in temperature, and so it no longer remains the same specimen as the culture itself. To save the organism from drying out and evaporation, water is added to it periodically, but this changes the salinity of the sample. All these changes eventually lead to change in the sample’s natural behavior and end up killing them shortly.
This shortcoming of the light microscope is solved by the inverted one, because it allows users to observe an entire culture directly from a large container. This allows analyzing the sample without changing its natural behavior and so the results are more accurate.