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Fluorescence Microscopes are used in the study of both organic and inorganic matter. It uses the release of light by a stained substance that has taken in either light or other electromagnetic radiation. Fluorescence Microscopes use an extremely high intensity light to generate an image to illuminate the sample being studied. This release of light has a longer wavelength, which is what causes the fluorescence in the sample. Various stains are used in conjunction with the specimen in order to facilitate the fluorescing process.
Earlier types of fluorescence microscopes include the use of xenon, halogen, and the most commonly used today are with mercury bulbs - all powered by an external power supply. As technology constantly evolves, manufacturers have introduced alternative fluorescent light sources, including including Metal Halide and LED Fluorescence. They are more expensive but the bulbs have considerable more longevity, thus a savings in the long run.
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