This model features both transmitted and incident illumination advanced features and optics that is perfect for both brightfield and fluorescence applications. One model is available for phase contrast applications and comes with a Zernike-style phase contrast condenser with annulus rings. All models come standard with quadruple reversed nosepiece with positive click stops. The viewing head is Seidentnopf and all models come trinocular for either photo or video capabilities. There is a slide out main prism that facilitates maximum light through the head. All models have a graduated mechanical stage with 30 x 70mm movable range. The focusing mechanism is coaxial for precise focusing control. The upper illumination system is 100W HBO with a mercury light source and the lower illumination system is 6V 20W variable halogen (supplied with blue, green and neutral density filters). The model for phase contrast has a centerable field diaphragm for Kohler illumination.
Item # 1486FLi Fluorescence Microscopes, Professional Level – 1400FLi Series
- Professional Level
- Fluorescence, Phase, Brightfield, & Darkfield
- Precision Optics
The 1400FLi Series Fluorescence Microscopes are the flagship of the VanGuard line. Combining top performance with highly-advanced features and optics, the 1400FLi Series models produce fluorescence images of the utmost in clarity. Available with brightfield or phase contrast / brightfield / darkfield.
Viewing Head: Trinocular heads rotate 360° and are inclined at 30°. Both models feature interpupillary and dioptric adjustment. The heads feature a sliding main prism (20/80 split) to provide full-time imaging when the vertical tube is in use (80% of the image to the vertical tube and 20% to the viewing eyetubes).
Eyepieces: 10x Widefield (DIN) with a field number of 18.
Nosepiece: Quintuple, reversed, ball-bearing nosepiece with high-grade lubricant and positive stops. The nosepiece is reversed (inward-facing) to allow for easier manipulation of slides and to aid in keeping the objectives clean.
Objectives: Achromatic fluorescence objectives come standard on both models. Model 1482FLi features achromatic brightfield/fluorescence objectives. Model 1486FLi features plan achromatic phase contrast and achromatic brightfield/fluorescence objectives. All objectives are made to DIN standards and are coated.
Stage: Delivering a high level of fluid motion control and longevity, the stage measures 160 x 140 mm. Features a removable spring-clip slide holder and a chemical-resistant finish. Motion is controlled by a right-hand, low-position coaxial control and is driven by a rack and pinion system.
Focusing Movement: Coaxial, ultra low-position coarse and fine focus controls feature a 40mm focusing range and are graduated to 2 microns per division. Fitted with tension adjustment and safety auto stop.
Condenser: Model 1482FLi comes with a 1.25 N.A. Abbe condenser. Model 1486FLi comes with a 1.25 N.A. Zemike condenser with phase annulus rings for 10x, 20x, 40x, & 100x; also has settings for brightfield and darkfield work. All condensers are mounted on a rack and pinion focusing mechanism and feature spring-loaded centering knobs and an iris diaphragm with a swing-in filter holder.
Lower Illumination: 20W variable quartz halogen light source. Comes with blue, green (Model 1486FLi only), and dispersion filter. 0.25 A, 250 V (2 ea.) fuses.
Upper Illumination: Features a 100 W HBO mercury light source which is mounted to the rear and is protected by a sturdy, all-metal housing. Lamp is easily focused and centered with the front-mounted lamp viewer. External power supply provides constant, even light and features an electronic timer which automatically logs the amount of time the mercury lamp is in use. 8.0 A, 250 V fuses (2 ea.).
Fluorescence Filters: Features a 3-position (2 filter positions and 1 OFF position), sliding, filter cube assembly. Fluorescence models come standard with 2 broadband filters (blue & green). Custom fluorescence filters available on request.
Base: Stable 225 x 160 mm base fitted with anti-skid rubber feet.
Body: Cast-metal, ergonomic body with stain-resistant enamel finish.
Dimensions: 225 mm (L) x 160 mm (W) x 465 mm (H); 10.7 kg
|Optical System||VIOS Infinity-Corrected|
|Objective Magnification||4x, 10x, 40x, 100x (Oil)
10x, 20x, 40x, 100x (Oil)
|Objective Type||Plan Achromatic
Plan Achromatic Phase
|Viewing Head||30° Inclination; 360° Rotatable|
|Interpupillary Adjustment||55-75 mm|
|Dioptric Adjustment||-6 to +6|
|Camera Port||20/80 Split|
|Eyepieces||10x WideField; 18 mm F.D.|
|Nosepiece||5-Position; Reverse Pitch|
|Fluorescence/Brightfield Objectives (Plan Achromatic)||100x (1.25 NA, 0.25 mm W.D., 0.18 mm F.O.V.)
10x (0.25 NA, 8.0 mm W.D., 1.8 mm F.O.V.)
40x (0.65 NA, 0.40 mm W.D., 0.45 mm F.O.V.)
4x (0.10 NA, 17.0 mm W.D., 4.5 mm F.O.V.)
|Phase Contrast Objectives (Plan Achromatic)||10x (0.25 N.A., 8.0 mm W.D., 1.8 mm F.O.V.)
20x (0.40 NA, 0.50 mm W.D., 0.9 mm F.O.V.)
40x (0.65 NA, 0.40 mm W.D., 0.45 mm F.O.V.)
100x (1.25 NA, 0.25 mm W.D., 0.18 mm F.O.V.)
|Fluorescence Filter Cubes1||Blue Broadband
|Stage||Rectangular 160 x 140 mm Mechanical; Drop-Down, Right-Hand, Rack & Pinion X-Y Control, 75 x 50 mm Cross Travel Range; Removable Spring-Clip Slide Holder; Chemical-Resistant Finish|
|Focusing||Coaxial, Low Position, Coarse/Fine Controls; Tension Adjustment; Safety Auto-Stop; 40 mm Focusing Range; 0.002 mm Precision|
|Condenser||1.25 N.A. Abbe; Iris Diaphragm; Swing-Out Filter Holder; Rack & Pinion Focusing Control; 10x, 20x, 40x, 100x Zernike Phase Stops; Darkfield Stop; Telescoping Eyepiece For Phase Annuli Centering|
|Upper Illumination||100 W HBO Mercury|
|Lower Illumination||20 W Variable Quartz Halogen|
|Base||225 x 160 mm; Anti-Skid Rubber Feet|
|Body||Ergonomic; Stain-Resistant Enamel Finish|
|Dimensions||225 x 160 x 465 mm|
220 V Available On Request
|Warranty||Five Year Manufacture and Free One Year Extended, Total of Six Years|
|Supplied With||Dispersion Filter, Blue Filter, Green Filter, Telescoping Eyepiece, Spare 20 W Halogen Lamp, Spare 0.25A, 250 V Fuses (2 Ea.), Spare 8.0A, 250 V Fuses (2 Ea.), Dust Cover, & Operation Manual|
|1 Blue Broadband
(Excitation: 430-490 nm; Emission: 520+ nm; Dichroic: 500 nm) Intended Dyes: FITC, Acridine Orange, & Auramine
1400FLi User’s Manual